AFLP-PCR uses many of the same steps as RFLP, SSR, and RAPD, including restriction enzyme digestion of genomic DNA, PCR, and electrophoretic separation of DNA fragments. However, this method ...

Template fragments are generated by: (1) digestion of genomic DNA with a combination of the two restriction enzymes EcoRI and MseI (blue and red arrows represent EcoRI and MseI restriction enzyme ...

The following techniques are covered: genomic DNA isolation, restriction analysis of genomic and plasmid DNA, gel electrophoresis, Southern transfer of genomic DNA, non-radioactive DNA hybridization, ...

These tools include molecular marker techniques such as AFLP, RAPD, AFLP, and PCR amplified DNA sequences. The molecular tools allow detection of specific DNA fragments through successive generations, ...